Analysis Note

Positive ControlHepG2 cells

Negative Control293T cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~70 kDa PCK1 protein.

This Anti-PCK1 Mouse mAb (3E4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of PCK1.

Recognizes the ~70 kDa PCK1 protein in HepG2 cells.

Immunogen

Full-length, recombinant human PCK1 (aa 1-623), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)